Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Digitaria striate (?) cytorhabdovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.S. Greber, 1987.

Nomenclature

Acronym

DiSV (DSV)

ICTV decimal code

62.U.P.0.016

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Digitaria ciliaris, D. decumbens, D. sanguinalis, Brachiaria miliiformis - chlorotic spots and stripes.

Transmission

Transmitted by a vector; an insect; Sogatella kolophon; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Australia.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Avena sativa, Digitaria ciliaris, Digitaria decumbens, Dinebra retroflexa, Eleusine indica, Hordeum vulgare, Lolium vulgare.

Diagnostically insusceptible host species

Echinochloa colona, Zea mays and Oryza sativa.

Maintenance and propagation hosts

Digitaria ciliaris, D. decumbens.

Assay hosts (Local lesions or Whole plants)

Digitaria ciliaris (W).

Susceptible host species

• Avena sativa
• Brachiaria miliiformis
• Digitaria ciliaris
• Digitaria decumbens
• Digitaria sanguinalis
• Dinebra retroflexa
• Eleusine indica
• Hordeum vulgare
• Lolium multiflorum
• Lolium vulgare

Insusceptible host species

• Chloris gayana
• Echinochloa colona
• Leptochloa filiformis
• Oryza sativa
• Sorghum bicolor
• Zea mays

Families containing susceptible hosts

• Gramineae (10/16)

Families containing insusceptible hosts

• Gramineae (6/16)

Sources of host-range data

Greber (1979).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Greber: for nucleocapsids extract Digitaria ciliaris in 0.1 M phosphate and thioglycollate, pH 7.6. Clarify in 25% CHCl₃ + 5% butanol. Purify and concentrate by high speed and sucrose gradient centrifugation.

Particle morphology

Virions rhabdo- or bullet-shaped; enveloped; with a clear modal length; of 280 nm; 55 nm wide. Axial canal obvious; 14 nm in diameter. Basic helix obvious; pitch of basic helix 4.2 nm.

Features of proteins

M_r 50000; N protein. Virion proteins glycosylated; are Only the G-protein.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll, phloem and companion cells; in cytoplasm. Inclusions absent from infected cells.

Taxonomy and relationships

Possible Cytorhabdovirus: Rhabdoviridae

Virus(es) with serologically related virions

Barley yellow striate mosaic and maize sterile stunt viruses.

Virus(es) with serologically unrelated virions

American wheat striate mosaic, cereal chlorotic mottle, cynodon rhabdovirus, festuca rhabdovirus, lettuce necrotic yellows and maize mosaic viruses.

Comments and References

References

• Greber, R.S. (1972). Aust. Pl. Path. Soc. Newsl. 1: 27.
• Greber, R.S. (1976). Aust. Pl. Path. Soc. Newsl. 5: 228.
• Greber, R.S. (1979). Aust. J. agric. Res. 30: 43.
• Greber, R.S. (1984). In: Current Topics in Vector Research, Vol. 2, p. 141; ed. K. Harris. Praeger Scientific, New York.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.