Descriptions and Lists from
the VIDE Database
Data collated by R.G. Milne, 1984.
virus del nanismo ruvido del mais.
type strain (TS),
cereal tillering disease strain (CTD) (Boccardo and Milne, 1984; Lindsten and
Gerhardson, 1971), mal de Rio Cuarto (MR4) (Boccardo and Milne, 1984; Nome et
ICTV decimal code
Host range and symptoms First reported
in Zea mays; from Israel; by Harpaz (1959).
Natural host range and symptoms
- Avena sativa (CTD strain), Cynodon dactylon, Digitaria
sanguinalis, Echinochloa crus-galli, Hordeum vulgare (CTD strain), Lolium
perenne, Setaria verticillata, Triticum aestivum (CTD strain), Zea
mays (dent, not flint cultivars) - stunting, dark green colour, enations
on veins of leaves and sheaths, longitudinal splitting of roots, flowers
Transmitted by a vector; an insect;
Delphacodes propinqua, Dicranotropis hamata, Laodelphax striatellus,
Javasella pellucida, Sogatella vibix; Delphacidae. Transmitted in a
persistent manner. Virus retained when the vector moults; multiplies in the
vector; transmitted congenitally to the progeny of the vector (one unconfirmed
report); transmitted by mechanical inoculation (injection; but very
inefficient); not transmitted by grafting; not transmitted by contact between
plants; not transmitted by seed; not transmitted by pollen.
Spreads in Argentina, the former
Czechoslovakia, France, Israel, Italy, Norway, Spain, Sweden, and the former
Experimental host range
Few (<3) families susceptible.
Diagnostically susceptible host species and symptoms
- Hordeum vulgare cv. Delesa - stunting.
mays American yellow dent hybrids; Wisconsin 641 AA - stunting, dark
green colour, pale yellow enations on abaxial surface of leaves and leaf
Diagnostically insusceptible host species
Saccharum spp., Sorghum halepense (susceptible to the Chinese
virus), Stipa bromoides, probably all non-Gramineae.
Maintenance and propagation hosts
Triticum aestivum, Zea mays.
Assay hosts (Local lesions or Whole plants)
Hordeum vulgare (W), Triticum aestivum (W), Zea mays (W).
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Sources of host-range data
and Lovisolo (1977).
Properties of particles in sap
contains few virions, or contains many virions (few virions in whole leaf sap,
many in sap from enations). Electron microscopy: for whole virions avoid neutral
PTA, use 2% aqueous UA. No special treatment for subvirions.
al. (1973); Boccardo and Milne (1981).
Virions isometric; not enveloped; 70
nm in diameter; rounded in profile, or angular in profile; with a conspicuous
Two sedimenting components in
purified preparations (whole and subvirions); sedimentation coefficient 400
S (for subviral non-infectious virions).
Virions 0 % lipid.
Genome consists of RNA; double-stranded; linear. Total genome size 26.169
kb. Genome of 10 parts (named S1-10); largest (or only) genome part the
largest 3.9 kb (pairs); the 2nd largest 3.2 kb (pairs); the 3rd largest 3.15 kb
(pairs); the 4th largest 3.15 kb (pairs); the 5th largest 3.1 kb (pairs); the
6th largest and other parts 2.293 kb (pairs; also 2.198, 1.735, 1.728 and 1.715
kb pairs). Genomic nucleic acid isolated by Boccardo and Milne (1975).
Additional factor required for infectivity; proteins from the virions; isolated
nucleic acid is not infectious.
Sequence database accession code(s)
Em(40)_un:MRDVS6 Gb(84)_un:MRDVS6 Maize rough dwarf virus (MRDV) mRNA for
genomic segment S6. 3/91 2,193bp. 1 sequence.
Features of the genome
Non-genomic nucleic acid
not found in the virions.
Features of proteins
Virion protein(s) six;
Mr of the largest 139000. Mr of 2nd largest 125000.
Mr of 3rd largest 123000. Mr of 4th largest 111000.
Mr of 5th largest 97000. Mr of 6th largest and smaller,
64000. Method of preparation: Boccardo and Milne (1975).
Genome replicates in cytoplasmic viroplasms.
Coat protein mRNA translated in the cytoplasm. Replication does not
depend on a helper virus.
Virions found in phloem enations; in
cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm,
or viroplasms (containing immature virions), or unusual in shape; tubular
structures some 100 nm diameter; they contain virions. Other cellular changes:
cell wall proliferation and cell enlargement.
Virus(es) with serologically related virions
The virus is so closely related to rice black streaked dwarf virus,
that it may be the same species, it is more distantly related to pangola stunt
Virus(es) with serologically unrelated virions
Oat sterile dwarf, rice ragged stunt and sugarcane Fiji
The virus has been
reported from China, but it may be that the virus was incorrectly identified,
and was rice black-streaked dwarf virus, which is common in the region.
- Boccardo, G. and Milne, R.G. (1975).
Virology 68: 79.
- Boccardo, G. and Milne, R.G. (1981). J.
virol. Meth. 3: 109.
- Boccardo, G. and Milne, R.G. (1984).
CMI/AAB Descr. Pl. Viruses No. 294, 7 pp.
- Conti, M. (1984). In:
Current Topics in Vector Research; ed. K.F. Harris. Praeger Scientific,
- Francki, R.I.B. and Boccardo, G. (1983). In: The
Reoviridae. p. 155; ed. K.W. Joklik. Plenum Press, New York.
R.I.B., Milne, R.G. and Hatta, T. (1984). In: An Atlas of Plant Viruses.
Vol. 1. CRC Press, Boca Raton, Florida.
- Harpaz, I. (1959). Nature,
Lond. 184: 77.
- Lindsten, K. and Conti, M. (1977). Annls.
Phytopath. 9: 30.
- Lindsten, K. and Gerhardson, B. (1971). Nat.
Swed. Inst. Pl. Prot. Count. S. 14: 285.
- Lovisolo, O. (1971).
CMI/AAB Descr. Pl. Viruses No. 72, 4 pp.
- Marzachi, C., Boccardo, G.
and Nuss, D.L. (1991). Virology 180: 518.
- Milne, R.G. and
Lovisolo, O. (1977). Adv. Virus Res. 21: 267.
- Milne, R.G. and
Luisoni, E. (1977). Virology 80: 12.
- Milne, R.G., Conti, M.
and Lisa, V. (1973). Virology 53: 130.
- Nome, S.F., Lenardon,
S.L., Raju, B.C., Laguna, I.G., Lowe, S.K. and Docampo, D. (1981). Phytopath.
Z. 101: 7.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
Dallwitz, Paine and Zurcher (1993)
should also be cited.
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