Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Rice hoja blanca tenuivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by F.J. Morales, 1984. Revised 1989.

Nomenclature

Synonyms

echinochloa hoja blanca virus (Falk et al., 1987).

Acronym

RHBV

Strains

echinochloa strain (Morales and Niessen, 1985).

ICTV decimal code

69.0.1.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Oryza sativa, wild Oryza spp. - systemic necrosis, systemic chlorotic striping in early infections.

Transmission

Transmitted by a vector; an insect; Sogatodes orizicola; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.

Ecology and control

Studies reported by Everett and Lamey (1969); Lamey (1967).

Geographical distribution

Spreads in the South and Central American region; Colombia, Costa Rica, Cuba, the Dominican Republic, Ecuador, El Salvador, Honduras, Nicaragua, Panama, Peru, and Venezuela (and elsewhere in the Caribbean). Found, but with no evidence of spread, in the U.S.A.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Avena sativa, Digitaria spp., Hordeum vulgare, Leptochloa spp., Oryza sativa cv. Bluebonnet-50, Secale cereale, Triticum aestivum - chlorotic stripes.

Diagnostically insusceptible host species

host range restricted to Gramineae.

Maintenance and propagation hosts

purified from susceptible Oryza sativa cultivars only.

Assay hosts (Local lesions or Whole plants)

susceptible Oryza sativa cultivars (W).

Susceptible host species

• Avena sativa
• Digitaria
• Hordeum vulgare
• Leptochloa
• Oryza sativa
• Secale cereale
• Triticum aestivum

Families containing susceptible hosts

• Gramineae (7/7)

Sources of host-range data

Lamey et al. (1964).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Morales and Niessen (1983).

Particle morphology

Virions filamentous; not enveloped; with no clear modal length; 3 nm wide.

Physical properties

One sedimenting component in purified preparations to two sedimenting components in purified preparations (in caesium salts), or one sedimenting component in purified preparations to three sedimenting components in purified preparations (in sucrose). Density 1.288 g cm^-3 in CsCl. Isoelectric point pH 4.5.

Biochemical properties

Virions contain 12 % nucleic acid (determined from A_260/280).

Genome consists of RNA; single-stranded; linear. Total genome size 17.59 kb. Genome of four parts; largest (or only) genome part 9.8 kb; the 2nd largest 3.5 kb; the 3rd largest 2.3 kb; the 4th largest 1.99 kb.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• L07940 Em(40)_vi:HBVMNS3NP Gb(84)_vi:HBVMNS3NP Rice hoja blanca virus non-structural protein 3 (NS-3) and nucleoprotein (N-protein) genes, c
• L14952 Em(40)_vi:HBVNS4VC Gb(84)_vi:HBVNS4VC Rice hoja blanca virus nonstructural protein (NS4) and (NS4vc) genes, complete cds's. 3/94 1.
• L39989 Em(43)_vi:Hbvpv2a Gb(89)_vi:Hbvpv2a Rice hoja blanca virus membrane associated protein (pv2) gene, complete cds. 3/95 988bp.

Features of proteins

Virion protein(s) one; M_r 34000.

Virus-coded non-virion proteins isolated (Gingery et al. (1981)); one protein found. M_r 17500.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are amorphous X-bodies; they contain virions. Other cellular changes: disorganisation of chloroplasts, mitochondria and nuclei.

Taxonomy and relationships

Tenuivirus

Virus(es) with serologically related virions

Echinochloa hoja blanca virus.

Virus(es) with serologically unrelated virions

Maize stripe and rice stripe viruses.

Best tests for diagnosis

Rice hoja blanca is the only virus known to affect rice in the Americas.

Comments and References

References

• Everett, T.R. and Lamey, H.A. (1969). In: Viruses, Vectors, and Vegetation; ed. K. Maramorosch. Interscience, New York.
• Flak, B.W., Morales, F.J., Tsai, J.H. and Niessen, A. (1987). Phytopathology 77: 196.
• Garcés (1940). In: Projects in Plant Pathology at the Palmira Experimental Station, unnumbered report to the Ministry of Economy, Bogota.
• Gingery, R.E., Nault, L.R. and Bradfute, O.E. (1981). Virology 112: 99.
• Lamey, H.A. (1967). In: The Virus Diseases of the Rice Plant, 354 pp. John Hopkins, Baltimore.
• Lamey, H.A., McMillan, W.W. and McGuire, J.U. (1964). Phytopathology 54: 536.
• Morales, F.J. and Niessen, A.I. (1983). Phytopathology 73: 971.
• Morales, F.J. and Niessen, A.I. (1985). AAB Descr. Pl. Viruses No. 299, 4 pp.
• Ramirez, B.C., Macaya, G., Calvert, L.A. and Haenni, A.-L. (1992). J. gen. Virol. 73: 1457.
• Ramirez, B.C., Lozano, I., Constantino, L.-M., Haenni, A.-L. and Calvert, L.A. (1993). J. gen. Virol. 74: 2463.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.