Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Rice ragged stunt oryzavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by G. Boccardo, 1984.

Nomenclature

Synonyms

rice infectious gall virus.

Acronym

RRSV

ICTV decimal code

60.0.9.0.003

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Oryza latifolia, O. nivara, O. sativa - stunting, enations on veins of leaves and leaf sheaths, ragged leaves, flower suppression.

Transmission

Transmitted by a vector; an insect; Nilaparvata lugens; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission; not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Bangladesh, China, India, Indonesia, Japan, Malaysia, the Philippines, Sri Lanka, Taiwan, and Thailand.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Oryza sativa cv. Taichung Native (TN-1) - pale spindle-shaped enations on abaxial surfaces of leaves and leaf sheaths, twisted and ragged leaves, dark green colour, suppressed flowering, infected plants remain green and appear grassy after healthy plants have senesced.

Diagnostically insusceptible host species

Triticum aestivum, Saccharum spp.

Maintenance and propagation hosts

Oryza sativa cv. Taichung Native (TN-1).

Assay hosts (Local lesions or Whole plants)

Oryza sativa (W).

Susceptible host species

• Hordeum vulgare
• Oryza latifolia
• Oryza nivara
• Oryza sativa
• Zea mays

Insusceptible host species

• Saccharum officinarum
• Triticum aestivum

Families containing susceptible hosts

• Gramineae (5/7)

Families containing insusceptible hosts

• Gramineae (2/7)

Sources of host-range data

Hibino (1979); Shikata et al. (1979).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions (whole leaf sap), or contains many virions (in sap from enations).

Purification method

Milne et al. (1982).

Particle morphology

Virions isometric; not enveloped; 65 nm in diameter; angular in profile; without a conspicuous capsomere arrangement (but with 12 "B" type spikes 8-10 nm long).

Physical properties

One sedimenting component in purified preparations.

Biochemical properties

Genome consists of RNA; double-stranded. Total genome size 26.66 kb (pairs). Genome of 10 parts (named S1-10); largest (or only) genome part the largest 3.9 kb (pairs); the 2nd largest 3.9 kb (pairs); the 3rd largest 3.8 kb (pairs); the 4th largest 3.8 kb (pairs); the 5th largest 2.75 kb (pairs); the 6th largest and other parts 2.3 kb (pairs; also 1.95, 1.90, 1.20 and 1.16 kb pairs). Additional factor required for infectivity; as only the whole virion is infective.

NCBI sequence data (Entrez search)

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) 8; M_r 125000. M_r of 2nd largest 97000. M_r of 3rd largest 66000. M_r of 4th largest 64000. M_r of 5th largest 48000. M_r of 6th largest and smaller, 43000 (also 36000 and 32000).

Replication

Genome replicates in cytoplasmic viroplasms. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in phloem enations; in cytoplasm. Inclusions present in infected cells; are viroplasms; they contain virions. Other cellular changes: cell enlargement and sometimes cell wall proliferation.

Taxonomy and relationships

Oryzavirus: Reoviridae (the type species)

Virus(es) with serologically unrelated virions

Sugarcane Fiji disease, maize rough dwarf, pangola stunt, rice black-streaked dwarf, oat sterile dwarf, rice dwarf, rice gall dwarf and clover wound tumor viruses.

Comments and References

References

• Boccardo, G. and Milne, R.G. (1980). Intervirology 14: 57.
• Boccardo, G. and Milne, R.G. (1984). CMI/AAB Descr. Pl. Viruses No. 294, 7 pp.
• Chen, C.C., Hsu, Y.H., Chen, M.J., and Chin, R.J. (1989). Intervirology 30: 278.
• Hibino, H. (1979). Rev. Pl. Protect. Res. 12: 98.
• Hibino, H. and Kimura, I. (1982). Phytopathology 72: 656.
• Hibino, H., Saleh, N. and Roechan, M. (1979). Ann. Phytopath. Soc. Japan 45: 228.
• Hibino, H., Roechan, M., Sudarisman, S. and Tantera, D.M. (1977). Contr. Centr. Res. Inst. Agric. Bogor No. 35: 15.
• Lee, S.Y., Uyeda, I. and Shikata, E. (1987). intervirology 27: 189.
• Ling, K.C. (1977). Int. Rice Res. Newsl. 2: 6.
• Ling, K.C., Tiongco, E.R., Aguiero, V.M. and Cabauatan, P.Q. (1978). Int. Rice Res. Paper Series 16, 25 pp.
• Luisoni, E., Milne, R.G. and Roggero, P. (1982). Plant Dis. 66: 929.
• Milne, R.G. (1980). Intervirology 14: 331.
• Milne, R.G., Luisoni, E. and Ling, K.C. (1979). Pl. Dis. Reptr 63: 445.
• Milne, R.G., Boccardo, G. and Ling, K.C. (1982). CMI/AAB Descr. Pl. Viruses No. 248, 5 pp.
• Shikata, E., Senboku, T., Kamjaipai, K., Chou, T., Tiongco, E.R. and Ling, K.C. (1979). Ann. Phytopath. Soc. Japan 45: 436.
• Shikata, E., Leelopang, Tiongo, E.R. and Ling, K.C. (1977). Int. Rice Res. Newsl. 2: 7.
• Yan, J., Kudo, H., Uyeda, I., Lee, S.Y. and Shikata, E. (1992). J. gen. Virol. 73: 785.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.