Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Tobraviruses

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Illustrations

Nomenclature

ICTV reference

ICTV decimal code 72.0.1.

Definitive species

• Pea early browning tobravirus
• Pepper ringspot tobravirus
• Tobacco rattle tobravirus

Tentative species

• Harts tongue fern (?) tobravirus

Host range and symptoms

Natural host range and symptoms

Symptoms persist, or vary seasonally (1), or disappear soon after infection (1).

Transmission

Transmitted by a vector (2/2); a nematode (2/2); Trichodoridae (2/2). Virus transmitted by mechanical inoculation; transmitted by grafting (2/2); not transmitted by contact between plants (2/2); transmitted by seed (2/2).

Experimental host range

Many (>9) families susceptible (2), or several (3-9) families susceptible (1), or few (<3) families susceptible (1).

Susceptible host species

• Allium cepa (1)
• Amaranthus caudatus (1)
• Amaranthus retroflexus (1)
• Antirrhinum majus (1)
• Arachis hypogaea (1)
• Avena sativa (1)
• Bellis perennis (1)
• Beta vulgaris (2)
• Bidens pilosa (1)
• Brassica campestris (1)
• Brassica campestris ssp. napus (1)
• Brassica campestris ssp. pekinensis (1)
• Brassica juncea (1)
• Calendula officinalis (1)
• Capsella bursa-pastoris (1)
• Capsicum annuum (2)
• Catharanthus roseus (2)
• Cheiranthus cheiri (1)
• Chenopodium album (2)
• Chenopodium amaranticolor (3)
• Chenopodium foetidum (1)
• Chenopodium murale (2)
• Chenopodium quinoa (3)
• Coriandrum sativum (1)
• Cucumis melo (1)
• Cucumis sativus (3)
• Cynara scolymus (1)
• Datura stramonium (2)
• Glycine max (2)
• Gomphrena globosa (3)
• Gypsophila elegans (1)
• Helianthus annuus (1)
• Hyacinthus (1)
• Hyoscyamus niger (1)
• Lactuca sativa (1)
• Lathyrus odoratus (2)
• Linum usitatissimum (1)
• Lobelia erinus (1)
• Lupinus mutabilis (1)
• Lycopersicon esculentum (2)
• Lycopersicon pimpinellifolium (1)
• Melilotus albus (1)
• Momordica balsamina (1)
• Myosotis sylvatica (1)
• Narcissus pseudonarcissus (1)
• Nicandra physalodes (1)
• Nicotiana benthamiana (2)
• Nicotiana clevelandii (3)
• Nicotiana debneyi (1)
• Nicotiana glutinosa (3)
• Nicotiana megalosiphon (1)
• Nicotiana rustica (2)
• Nicotiana sylvestris (1)
• Nicotiana tabacum (3)
• Nicotiana × edwardsonii (1)
• Ocimum basilicum (2)
• Pellaea falcata (1)
• Petunia × hybrida (3)
• Phaseolus vulgaris (3)
• Phyllitis scolopendrium (1)
• Phytolacca americana (1)
• Pisum sativum (3)
• Polystichum falcatum (1)
• Pteris `Childsii' (1)
• Pteris cretica var. albo-lineata (1)
• Raphanus sativus (1)
• Ricinus communis (1)
• Salvia splendens (1)
• Senecio vulgaris (1)
• Solanum melongena (1)
• Solanum nigrum (1)
• Solanum tuberosum (1)
• Spinacia oleracea (3)
• Stellaria media (1)
• Tetragonia tetragonioides (2)
• Trifolium hybridum (1)
• Trifolium pratense (1)
• Trifolium repens (1)
• Tropaeolum majus (2)
• Tulipa (1)
• Vicia faba (2)
• Vicia villosa (1)
• Vigna unguiculata (1)
• Viola arvensis (1)

Insusceptible host species

• Allium cepa (1/3)
• Avena sativa (1/3)
• Beta vulgaris (1/3)
• Brassica campestris ssp. rapa (1/3)
• Citrus medica (1/3)
• Cucumis sativus (1/3)
• Cucurbita pepo (1/3)
• Datura ferox (1/3)
• Datura metel (1/3)
• Datura stramonium (1/3)
• Daucus carota (1/3)
• Dianthus barbatus (1/3)
• Dianthus caryophyllus (1/3)
• Fragaria vesca (1/3)
• Gomphrena globosa (1/3)
• Hordeum vulgare (2/3)
• Lactuca sativa (1/3)
• Lycopersicon esculentum (1/3)
• Matthiola incana (1/3)
• Medicago sativa (1/3)
• Nicotiana clevelandii (1/3)
• Nicotiana glutinosa (1/3)
• Nicotiana tabacum (1/3)
• Phlox drummondii (1/3)
• Physalis floridana (1/3)
• Rumex acetosa (1/3)
• Secale cereale (1/3)
• Sinningia speciosa (1/3)
• Solanum tuberosum (1/3)
• Trifolium incarnatum (1/3)
• Trifolium pratense (1/3)
• Trifolium repens (1/3)
• Triticum aestivum (2/3)
• Vicia faba (1/3)
• Vigna unguiculata (1/3)
• Zea mays (1/3)
• Zinnia elegans (1/3)

Families containing susceptible hosts

• Alliaceae (1)
• Amaranthaceae (3)
• Amaryllidaceae (1)
• Apocynaceae (2)
• Aspidiaceae (1)
• Aspleniaceae (1)
• Boraginaceae (1)
• Campanulaceae (1)
• Caryophyllaceae (1)
• Chenopodiaceae (3)
• Compositae (2)
• Cruciferae (1)
• Cucurbitaceae (3)
• Euphorbiaceae (1)
• Gramineae (1)
• Hyacinthaceae (1)
• Labiatae (2)
• Leguminosae-Papilionoideae (3)
• Liliaceae (1)
• Linaceae (1)
• Phytolaccaceae (1)
• Pteridaceae (1)
• Scrophulariaceae (1)
• Sinopteridaceae (1)
• Solanaceae (3)
• Tetragoniaceae (2)
• Tropaeolaceae (2)
• Umbelliferae (1)
• Violaceae (1)

Families containing insusceptible hosts

• Alliaceae (1/3)
• Amaranthaceae (1/3)
• Caryophyllaceae (1/3)
• Chenopodiaceae (1/3)
• Compositae (2/3)
• Cruciferae (2/3)
• Cucurbitaceae (2/3)
• Gesneriaceae (1/3)
• Gramineae (2/3)
• Leguminosae-Papilionoideae (2/3)
• Polemoniaceae (1/3)
• Polygonaceae (1/3)
• Rosaceae (1/3)
• Rutaceae (1/3)
• Solanaceae (3/3)
• Umbelliferae (1/3)

Physical and biochemical properties

Properties of particles in sap

TIP: 74-80.33-85 °C. LIV: 40-90-183 days. DEP: log₁₀ minus 5-6 (2/3). Infectivity of sap not changed by treatment with di-ethyl ether (2/2). Leaf sap contains few virions (3/3).

Particle morphology

Virions rod-shaped; not enveloped (3/3); usually straight; with a clear modal length; of 46-158.3-320 nm; 21-21.75-22 nm wide. Axial canal obvious (3), or obscure (1); 4 nm in diameter. Basic helix obvious (3), or obscure (1); pitch of basic helix 2.5 nm.

Physical properties

Two sedimenting components in purified preparations (3/3); sedimentation coefficient 286-297-305 S; of the other(s) 163-186.5-210 S. Density 1.306-1.31-1.324 g cm^-3 in CsCl. Density 1.27 g cm^-3 in sucrose. A₂₆₀/A₂₈₀ ratio 1.1-1.137-1.15.

Biochemical properties

Virions contain 5 % nucleic acid; 95 % protein; 0 % lipid.

Genome consists of RNA (3/3); single-stranded (3/3); linear (3/3). Total genome size 9.09-10.63-12 kb. Genome of two parts (3/3); largest (or only) genome part 7.272-7.557-7.9 kb; the 2nd largest 1.818-3.076-4.242 kb. Base composition 24-24.7-25.5 % G; 26-27.65-29.5 % A; 16.5-17.27-18 % C; 28.5-30.37-32 % U. 5´ terminus of RNA has a methylated nucleotide cap (2/2). Infectivity retained when deproteinised with proteases (2/2); retained when deproteinised with phenol or detergent (2/2). Poly A region absent (2/2). Additional factor not required for infectivity (2/2). Genome has no tRNA-like activity (1/1).

NCBI sequence data (Entrez search)

Features of the genome

Non-genomic nucleic acid found in the virions (2/2); is subgenomic mRNA (2/2). Sub-genomic mRNA found in infected cells (2/2).

Features of proteins

Virion protein(s) one (3/3); M_r 21000-22660-24000.

Virus-coded non-virion proteins isolated (1/2), or identified by genomic sequence analysis (1/2); two proteins found (1/2), or three proteins found (1/2). M_r 169900-178400-186900. M_r of 2nd largest 139900-140900-141900. M_r of 3rd 30000.

Replication

Genome replicates in cytoplasm (1/1). Replication does not depend on a helper virus (2/2).

Cytopathology

Virions found in cytoplasm (2/2). Inclusions present in infected cells (2/2); are amorphous X-bodies (1/2), or unusual in shape (1/2); they contain virions (1/1).

Taxonomy and relationships

No data available.

Illustrations

Electron micrograph.

---

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.

Please send comments, corrections and suggestions to: vide-manager@biology.anu.edu.au